ISOLATION AND IDENTIFICATION OF SOME LOCAL DATE USING GEL FILTRATION CHROMATOGRAPHY & ELECTROPHORESIS
Basrah Journal For Date Palm Research,
Volume 4, Issue 1, Pages 87-110
AbstractThe present study is concerned with studying physical & chemical examinations for
dates & seeds of some local varieties such as: Hallawi, Sayer & Barhee. The results
of the statistical analysis about the physical properties showed that there were high
significant differences among varieties in weight, diameter, size, flesh weight & seed
weight characters, but the date length did not appear any significant differences
among the varieties.
T he results of the chemical composition of destoned dates showed that Barhee
variety was highest in content of moisture & fat whom reached 31.43 & 0.6%
respectively & with high significant differences compare with varieties of Hallawi &
Sayer, while Sayer variety showed higher content of ash which reached 2.3%, while
protein content did not show any significant differences among the varieties.
Moreover, the results of the protein extraction by different solvents for date
showed that the water soluble proteins (albumins) was highest which was about 46.28
- 65.07%, while was lower to salt soluble proteins (globulins) which was about 4.04 -
6.03% from the total amount of proteins.
G el filtration for water soluble proteins which extracted from date showed that
existence three major peaks, while salt soluble proteins showed existence two major
peak with one minority peak between them, for all varieties. In case of alcohol
soluble proteins (prolamins) demonstrate existence small peak followed by one
majority big peak, while alkali soluble proteins (glutenins) showed one majority big
peak between two small peaks.
Electrophoresis studies showed that the salt soluble proteins extracted from date:
Hallawi, Sayer & Barhee, appearance three major bands whom were molecular
weights about 61000-62400 Dalton for first band, 27600-37500 Dalton for second
band & 18200-23500 Dalton for third band.
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