Regeneration of date palm (Phoenix dactylifera L.) CV. Sherafy from different apical explants In vitro
Basrah Journal For Date Palm Research,
Volume 6, Issue 1, Pages 64-80
AbstractThis study has been performed to determine the response of apical
sources [shoot tip ; Auxillary buds ; sub apical tissues ; leaf primordial and
flower buds] when cultivated on MS medium supplemented with 30 mg/L of
NAA ; 3 mg/L of 2ip and 3 g/L activated charcoal in inducing ; formation of
callus and production of somatic embryos on the same medium enriched with
10gm/L of NAA ; 2 mg/L of 2ip and 2 g/L activated charcoal and formation
organogenesis from callus on two media which were supplemented with 3 mg/L
2ip ; 1 mg/L 2ip and 1 mg/L NAA ; 250 mg/L charcoal for each media.
Results revealed that the flower buds surpassed in the time requested for
callus estabelish ment and the plant sources ratio which produced the callus, with
significant difference than other explant, the sub apical tissue failed to produce
the callus also, the shoot tip treatment had the highest average of produced callus
than other explant. Statistical analysis proved that the difference between flower
buds and shoot tip was not significant in the parameter of the dry weight of
callus. Which cultivated in ms medium supplemented with 10 gm/L of NAA
after two months from culturing, while the dry weight decreased significantly in
leaf primordial than other explant.
Flower buds treatment had the optimam periode to estabelish the
cylinderial embryos and number in comparision with other explant while the
longest periode recorded in leaf primordial treatment.
Resulted showed that the callus cultured on the medium supplemented
with 3 mg/L of 2ip decreased significantly in the requested periode for
organogenesis growth which were (186 and 213.25) days respectively.
The interaction between (shoot tip and 3 mg/L of 2-ip) was surpassed the
explant of shoot tip and 1 mg/L of 2ip in the requested time for appearance of
organogenesis, also some individual cylindrical somative embryos developed
from the callus cultured on the above media with some secondary embryos when
cultured on the same medium produced muttistem plantlets .
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